Quantitative NanoSIMS Imaging of Individual Vesicles to Investigate the Relation between Fraction of Chemical Release and Vesicle Size

We used correlative transmission electron microscopy (TEM) and nanoscale secondary ion mass spectrometry (NanoSIMS) imaging to quantify the contents of subvesicular compartments, measure partial release fraction 13C-dopamine in cellular nanovesicles as a function size. Three modes exocytosis comprise full release, kiss-and-run, release. The latter has been subject scientific debate, despite growing amount supporting literature. tailored culturing procedures alter vesicle size definitively show no correlation with In NanoSIMS images, content was indicated by presence isotopic dopamine, while vesicles which underwent were identified an 127I-labelled drug, they exposed during allowing entry into open prior its closing again. Demonstration similar fractions indicates that this mode is predominant across wide range sizes.